Figure 6.

Cytokine production by human peripheral blood mononuclear cells pre-incubated with different blocking molecules and stimulated with conidia from Sporothrix schenckii, Sporothrix brasiliensis, or Sporothrix globosa. Human peripheral blood mononuclear cells were pre-incubated for 60 min with 200 μg mL⁻¹ of laminarin, 10 μg mL⁻¹ of anti-mannose receptor, 10 μg mL⁻¹ of anti-TLR2, or 10 μg mL⁻¹ of anti-TLR2, and then challenged with conidia. For tumor necrosis factor-alpha (TNFα), interleukin 1β (IL-1β), interleukin 6 (IL-6), and interleukin 10 (IL-10), human peripheral blood mononuclear cells were co-incubated for 24 h with live conidia, whilst for interleukin 17 (IL-17) and interleukin 22 (IL-22) stimulation, the human cells were co-incubated with the UV-killed conidia. In all cases, the interactions were centrifuged, supernatants collected, and cytokines quantified by ELISA. Ab—antibody; S.—Sporothrix. None refers to the system where the human cells were pre-incubated only with PBS. Results are the median ± standard deviation from data generated with samples from eight donors, analyzed in duplicate. * p < 0.05 when compared with the cytokine level stimulated by PBS-pre-incubated cells.