Fig. 5. BioID and mass spectrometry reveal that TWIST2 interacts with chromatin remodeling enzymes in FN-RMS cells.

(A) Schematic of BioID performed in both RD and SMS-CTR cells independently to identify TWIST2-interacting proteins. A BirA* biotin ligase fused to TWIST2 (TWIST2-BirA*) labeled any proteins within 10 nm. After cell lysis and sonication, the biotin-labeled proteins were captured using streptavidin beads and identified by mass spectrometry. m/z, mass/charge ratio. (B) GO analysis of 137 high confidence TWIST2-interacting proteins that are common in RD and SMS-CTR cells. (C and D) Endogenous (C) SMARCA4 and SMARCB1 proteins and (D) CHD3 were pulled down after immunoprecipitation of Flag from lysates of RD cells infected with a lentivirus for Empty^3xFlag or TWIST2^3xFlag. GAPDH was used as the loading control. IP, immunoprecipitate; IB, immunoblot.