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. 2023 Apr 28;9(17):eadg5423. doi: 10.1126/sciadv.adg5423

Fig. 1. Alteration of skin barrier function in Zfp750−/− mice.

Fig. 1.

(A) Schematic representation of the strategy used for generating the KO mouse. Primers for genotyping are indicated with arrows. bp, base pair. (B) Real-Time PCR showing the absence of Zfp750−/− mRNA in the epidermis of Zfp750−/− mice. Data are normalized to β-actin and relative to WT. Bars represent the mean ± SD (n = 6 mice per genotype). (C) Gross phenotype of Zfp750−/− mice. Pictures of the WT and Zfp750−/− mice showing shiny and wrinkled skin and lack of eyelids (black arrow). (D) Weight at the birth of the indicated mice. (E) Kaplan Meier analysis of the post-natal death of Zfp750−/−mice. Zfp750−/−mice die within 20 hrs (WT, n = 2; ZFP750+/−, n = 9; ZFP750−/−, n = 5). (F) Hematoxylin and eosin staining of skin from WT and Zfp750−/−− mice showing the reduction of the epidermal thickness. Scale bar, 20 μm. CL, cornified layer; GL, granular layer; SL, spinous layer; BL, basal layer. (G) Zfp750−/− mice loss weight within 9 hours (h) after birth (*P < 0.05). (H) Skin permeability assay. Mice were stained with toluidine blue. E17.5, embryonic day 17.5; P0.5, postnatal day 0.5. (I) Inside-out skin barrier was assessed using EZ-Link sulfo-NHS-LC-biotin. Mice were injected intradermally into the skin of the back with EZ-Link sulfo-NHS-LC-biotin (10 mg/ml). Scale bar, 50 μm. ZO-1, zonula occludens-1; DAPI, 4′,6-diamidino-2-phenylindole.