Skip to main content
. 2023 Apr 28;9(17):eadg5423. doi: 10.1126/sciadv.adg5423

Fig. 3. Aberrant lipid composition in Zfp750−/−epidermis.

Fig. 3.

(A) Immunofluorescence analysis of glucosylceramide and ceramide (GlcCer/Cer) localization in the epidermis. Scale bars, 50 μm. (B) Nile red staining of the epidermal lipids showing a substantial decrease of the green fluorescence (neutral lipid species) in Zfp750−/− mice. Mutant mice showed a dot-like pattern (white arrowheads). Scale bars, 50 μm. (C) The intracellular content of lipid droplets is reduced in in vitro differentiated keratinocytes derived from Zfp750−/− mice. A representative micrograph of three independent experiments is shown. Scale bar, 50 μm. DD, Day of Differentiation. (D) Transmission electron micrographs showing abnormal lipid secretion (arrows) in ZFP750−/− mice (ii and iv) at the apical surface of the granular layer; persisting vesicle aggregates are found in the spaces among cells of SC (arrowheads). Scale bars, 200 nm (WT) and 500 nm (Zfp750−/−). A representative micrograph is shown (n = 4). (E) Western blot of CTSD showing a marked decrease in the precursor pro-CTSD in ZFP750−/− (β-actin, loading control). A representative immunoblot is shown (n = 2).