TABLE 4.

β-Galactosidase activity of plants inoculated with the wild type and flocculation mutants of A. brasilense containing a nifH-lacZ fusion^a

Strain	β-Galactosidase activityb
	2,4-D-treated plants	Plants without 2,4-D
Sp245 derivatives
Sp245 wild-type	100 ± 22	100 ± 17
Sp245-M4	67 ± 19	15 ± 6
Sp245-M5	83 ± 34	15 ± 6
Sp245-M6	98 ± 40	11 ± 2
Noninoculated plants	24 ± 13	1 ± 1
Sp7 derivatives
Sp7 wild type	100 ± 33	100 ± 35
Sp7-S	385 ± 150	110 ± 35
Sp72001	64 ± 5	65 ± 34
Sp72004	76 ± 18	54 ± 27
Sp7-PM35	108 ± 45	106 ± 53
Sp7-PM23	47 ± 15	68 ± 58
Noninoculated plants	17 ± 10	1.5 ± 1

^aPlants were inoculated with bacterial cells containing pAB358, a plasmid carrying a nifH-lacZ fusion. The data are averages of results for 10 plants. Standard errors are indicated. 

^bResults obtained using plants treated or not treated with 2,4-D (Miller units per minute per milligram of plant protein) are presented as percentages of the wild-type activity (Sp7 or Sp245, respectively).