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(A)The design principle of probe (TM-V) for ratiometric visualize mitochondrial viscosity in FRET mechanism. (B) The fluorescence intensity spectra of TM-V (10 μM) in the solvents of ethanol (E)–glycerol (G) mixtures under the exciting wavelength at 425 nm. (C) The bright field (a,e) and viscous fluorescence images (f–h) of TM-V (10 μM) in RAW 264.7 cells under normal stations (b–d) or treatment by PMA (f–h). Reproduced from Ref. [47] with permission of the Elsevier, copyright 2019.
