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. 2023 Apr 13;62(5):64. doi: 10.3892/ijo.2023.5512

Figure 4.

Figure 4

ZCCHC10 induces G1 arrest and apoptosis via p53 in AML cells. (A) Western blot analysis of ZCCHC10 protein expression in untreated cells (mock) and cells expressing empty vector (vec), ZCCHC10 cDNA (Zh10), scrambled shRNA (shNC) or ZCCHC10 shRNA (shZh10). (B) Cell viability was determined by a Cell Counting Kit-8 assay. (C) Diameter of colonies in triplicate experiments. (D) Distribution of the cell cycle in triplicate experiments, as determined by flow cytometry. (E) Apoptotic rate in triplicate experiments, as determined by flow cytometry. (F) Western blot analysis with the indicated antibodies. *P<0.05, **P<0.01 and ****P<0.0001. Representative images of colony formation and flow cytometry are shown in Figs. S1-S3. AML, acute myeloid leukemia; sh-, short hairpin; NC, negative control.