Figure 1.

Expression and characterization of recombinant UCD52 PC. The presence of the 5 components of the PC (gH, gL, pUL128, pUL130, and pUL131A) was confirmed by SDS-PAGE analysis using 4%–12% bis-tris polyacrylamide gel. The PC samples were analyzed at nonreducing (NR) and reducing (R) conditions. Protein bands were visualized with Coomassie blue staining, and the proteins corresponding to individual bands were identified by LC-MS/MS (see Supplementary Table 1). Abbreviations: LC-MS/MS, liquid chromatography with tandem mass spectrometry; PC, pentameric complex; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.