Figure 3.

Fluorescence microscopy of RhCMV UCD52 pp65-2 expressed from a transfected DNA vaccine vector. An anti-6×His antibody conjugated with FITC (green) was used to assay the presence of histidine-tagged UCD52 pp65-2 in mock transfected (right upper panel) or DNA vaccine vector transfected (right lower panel) COS-7 cells. Nuclei of the same cells were stained with DAPI (blue in the left panels). Scale bar = 100 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate; RhCMV, rhesus cytomegalovirus.