Skip to main content
. Author manuscript; available in PMC: 2024 Apr 20.
Published in final edited form as: Cell Chem Biol. 2023 Apr 7;30(4):362–382.e8. doi: 10.1016/j.chembiol.2023.03.006

Figure 1: Detection of CXCR3 C-terminal phosphopeptides using mass spectrometry.

Figure 1:

(A) Snake diagram of CXCR3 highlighting green putative C-terminal phosphorylation sites. (B) Schematic of experimental design of receptor phosphoproteomic experiment. (C) Singly, doubly, and triply phosphorylated CXCR3 C-terminal peptides identified through mass spectrometry. Identified phosphopeptides are noted in red. (D) Abundance of singly phosphorylated DSSWSETSEASYpSGL peptide measured in HEK293 cells following stimulation with vehicle control or CXCL9, CXCL10, or CXCL11 at 100 nM for 5 minutes. Mean ± SEM, n=2 technical replicates of 6 pooled biological replicates. (E) Diagram of designed CXCR3 phosphorylation-deficient receptor mutants of interest. *P<.05, by one-way ANOVA, Tukey’s post hoc analysis. See S1 for additional mass spectrometry data and signaling and expression data of CXCR3 phosphorylation deficient mutants.