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. 2023 Apr 28;9:138. doi: 10.1038/s41420-023-01437-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Relative cell viability of erastin (2, 3, 4, 5 μΜ) -stimulated fibroblasts. b Protein level of GPX4 after 5 μΜ erastin treatment. c Quantification of GPX4 immunoblotting in b. d, f Intracellular ROS and LPO level in fibroblasts with the indicated treatment. Scale bar in d = 200 μm, Scale bar in f = 100 μm. e, g Quantification of ROS and LPO level in d and f. Data represent the mean ± SEM of triplicates. *P < 0.05; **P < 0.01; ns nonsignificant, NF normal fibroblast, SF senescent fibroblast, CTCF corrected total cell fluorescence.