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. 2023 Apr 28;9:138. doi: 10.1038/s41420-023-01437-7

Fig. 4. Overexpression of NCOA4 repaired the ferritinophagy in senescent fibroblast.

Fig. 4

a, b The mRNA and protein level of NCOA4 in fibroblasts to detect the transfection efficiency of NCOA4. c Quantification of NCOA4 immunoblotting in b. d Immunofluorescence staining of NCOA4 in NF and SF after transfected NCOA4 plasmid. Scale bar = 100 μm. e Quantification of NCOA4 immunofluorescence staining in d. f Immunoblot of FTH1 in SF with the indicated treatment. i Quantification of FTH1 immunoblotting in f. g Intracellular free iron ions in fibroblasts with the indicated treatment. Scale bar = 50 μm. h Quantification of intracellular free iron ions in g. j, k Relative cell viability of NF and SF with erastin (5 μΜ) stimulation after transfection. Data represent the mean ± SEM of triplicates. **P < 0.01; ****P < 0.0001. DW diabetes wounds, NS normal skin, NF normal fibroblast, SF senescent fibroblast, CTCF corrected total cell fluorescence.