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. 2023 Apr 28;9:138. doi: 10.1038/s41420-023-01437-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a H&E staining of the wound indicated the healing situation on day 14. Scale bar = 200 μm (upper). b Immunohistochemistry staining of Ki67 in nDb and Db wound sections at day 14 after surgery. Scale bar = 50 μm. c Quantitative evaluation of the Ki67-positive cell ratio. d Prussian blue staining of the nDb and Db wounds at day 14 after surgery. Scale bar = 50 μm. e Quantitative evaluation of the Prussian blue-positive cell ratio. f Immunoblot of FTH1 and NCOA4 in wounds with the indicated treatment. g, h Quantification of FTH1 and NCOA4 immunoblotting in f. i, j The mRNA level of p16, FTH1, and NCOA4 were detected by real-time PCR in wounds with the indicated treatment. Data represent the mean ± SEM of triplicates. **P < 0.01; ***P < 0.001; ****P < 0.0001; ns nonsignificant, nDb non-diabetic, Db diabetic.