Figure 2.

WNT7A reduces adipogenesis of FAPs in a β-CATENIN-independent manner

(A) Experimental timeline of β-CATENIN expression and adipogenesis study. GM, growth media; ADM, adipogenic differentiation media.

(B) Representative immunofluorescence images of β-CATENIN-labeled FAPs treated with vehicle, WNT3A (200 ng/mL), and WNT7A (200 ng/mL) for 4 h. Scale bar: 100 μm.

(C) Representative immunofluorescence images of β-CATENIN-labeled FAPs treated with vehicle and WNT7A (200 ng/mL) for 48 h. Scale bar: 100 μm.

(D) Nuclear β-CATENIN intensity analyzed at the 4-h time point. >1,000 cells analyzed per condition in an automated manner from n = 3 biological replicates. One-way ANOVA with Tukey’s post-hoc analyses applied on the medians of biological donors. Mean ± SEM. ^∗p < 0.05; ^∗∗p < 0.01. n = 3. Colors represent biological replicates. Dotted line (at 10 a.u.) indicates the mean of WNT3A condition.

(E) Percentage of β-CATENIN⁺ nuclei analyzed at the 4-h time point. One-way ANOVA with Tukey’s post-hoc analyses. Mean ± SEM. ^∗p < 0.05. n = 3. Colors represent biological replicates.

(F) Nuclear β-CATENIN intensity analyzed at the 48-h time point. >440 cells analyzed per condition from n = 4 biological replicates. Two-tailed unpaired t test applied on the medians of biological donors. Mean ± SEM. n = 4. Colors represent biological replicates. Dotted line (at 10 a.u.) indicates the mean of WNT3A condition from (D).

(G) Percentage of β-CATENIN⁺ nuclei analyzed at the 48-h time point. Two-tailed unpaired t test. Mean ± SEM. n = 4. Colors represent biological replicates.

(H) Representative images of ORO-labeled cells treated with DMSO, PNU74654 (50 μM), and PNU74654 (50 μM) + WNT7A (200 ng/mL). Scale bar: 100 μm.

(I) Percentage of ORO⁺ cells with DMSO-, PNU74654-, and PNU74654 + Wnt7a-treated conditions. One-way ANOVA with Tukey’s post-hoc analyses. Mean ± SEM. ^∗p < 0.05; ^∗∗p < 0.01. n = 4. Colors represent biological replicates.