Figure 3.

Transcriptomic analysis of the differentiation of hPSCs (WA09) into neural crest and nociceptors

(A) PCA plot of RNA-seq experiment showing distinct molecular signatures at each timepoint.

(B) Gene enrichment analysis using ENRICHR and ARCHS4 database identify “sensory neuron” as the top category at days 28 and 56.

(C) Heatmap analysis showing stepwise differentiation of pluripotent cells into neural crest and nociceptors (n = 3). Data shown are from three different wells of one representative experiment.

(D–M) Time-course expression profile of different genes with importance in sensory neuron development.