Figure 3.

sTOs support isogenic T cell development

(A–D) FC plots (A and C) and quantification (B and D) of thymocytes in 1$°$ thymus and week 4 sTOs.

(E–H) CyTOF plots (E and G) and quantification (F and H) of thymocyte markers in 1$°$ thymus and week 4 sTOs (thymi, n = 6; sTOs, n = 4, 2 hPSC lines).

(I) qPCR analysis of CD4, CD8, and TdT in 1$°$ thymus, TEPs, and sTOs (thymi, n = 3; TEPs, n = 6–7, 4 hPSC lines; sTOs, n = 5–6, 4 hPSC lines).

(J and K) FC plots (J) and quantification (K) of CD4 and CD25 expression on stimulated T cells from week 4 sTOs (n = 3 ind. exp., 1 hPSC line).

Plots show percentage of the mean; data are shown as mean $\pm$ SEM; p values were determined by t test.