Skip to main content
. 2023 Apr 28;6(7):e202302023. doi: 10.26508/lsa.202302023

Figure S4. Rescue of the ciliogenic phenotype with xPHF8ΔC.

Figure S4.

(A) Representative immunocytochemistry images of tail bud stage embryos stained for acetylated α-tubulin (multiciliated cells). Injected reagents indicated on the left. Uninjected and injected sides indicated on top. Scale bars = 1 mm (whole embryo) and 200 μm (inserts), n = 3 biological replicates. (B) Schematic representation of the structure of PHF8 cDNAs: Full-length human PHF8 (hPHF8), wt Xenopus PHF8, and truncated Xenopus PHF8 (xPHF8ΔC). (A, C) Quantification of cilia staining in panel (A) in the four given conditions. (D) Confocal analysis of the rescue with xPHF8ΔC. Basal bodies in green, actin cap in red, ciliary axonemes in magenta, and DNA in blue. A mosaic injection scheme is used allowing KD and wt cells to be present in the same field of view (* = KD MCCs, wt = wild-type MCCs). Scale bars = 10 μm. (D, E) Quantification of confocal analysis in panel (D).