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. 2023 Mar 18;22(5):100532. doi: 10.1016/j.mcpro.2023.100532

Fig. 5.

Fig. 5

High expression of RAI14 promotes colon cancer cell migration.A–C, Western blot analyses show the effects of RAI14 on the expression of EMT marker proteins in colon cancer cell lines. D–F, effects of RAI14 on cell migration evaluated by transwell assays in response to RAI14 knockdown (E and F) and overexpression (D). Test method, Student’s t test, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05, for indicated comparisons. G–I, wound healing assays show cell migration of colon cancer cells after 48 h in response to RAI14 knockdown (G and H) and overexpression (I). Test method, Student’s t test, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05, for indicated comparisons. J, effects of RAI14 silencing on the formation of F-actin determined by immunofluorescence assays. Test method, Student’s t test, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05, for indicated comparisons. EMT, epithelial-mesenchymal transition.