FIGURE 5.

Silencing of miR‐183 reversed the suppression of proliferation and invasion of HTR‐8/SVneo cells and angiogenesis in vitro induced by IGF‐1 downregulation. A, The expression of miR‐183 after transfection of si‐IGF‐1 measured by RT‐qPCR. B, The viability of HTR‐8/SVneo cells measured by CCK‐8 assay after transfection of si‐IGF‐1 or miR‐183 inhibitor. C, The proliferation of HTR‐8/SVneo cells detected by colony formation experiments. D, The invasion of HTR‐8/SVneo cells detected by Transwell assay. E, The expression of VEGF measured by Western blot analysis. F, Detection of lumen formation detected by in vitro angiogenesis assay. All experiments were repeated three times. Data were measurement data and expressed as mean ± standard deviation. The independent sample t‐test was used for comparison between two groups. Comparisons among multiple groups were analysed by one‐way anova and followed by Tukey's post‐hoc test. * p < 0.05 vs. the si‐NC group, si‐NC + inhibitor‐NC and si‐IGF‐1 + inhibitor‐NC group. The cell experiment was repeated three times