Table 5.

Description of symbols in CellPathfinder

Method	Explanation
Neighbor	Associate a child object adjacent to a parent object.
Include	Associate a child object included in a parent object.
1 to 1	Link a single child object to a single parent object. Note: Since one cell has a nucleus, “Cytoplasm” must be associated with “Nucleus” using “1 to 1" (step 8f and Figure 2G).
1 to Many	Link several objects to a single parent object. Note: Since a lot of Gal3 dots exist in a cell, “Gal3” must be associated with “Cytoplasm” using “1 to Many” (step 8f and Figure 2G).

Explanations for the symbols in the setting “Link”. See step 8f.