Table 2.
Antibodies used for Immunoblotting and Immunostaining
| Target | Application | Sample Type | Source/Cat. # | Dilution |
|---|---|---|---|---|
| CatD | IB | Patient | Santa Cruz Biotechnology, sc- | 1:500 |
| IF | 6486 | 1:500 | ||
| CatD | IB | Mouse | R&D Systems, AF1029 | 1:500 |
| IF | 1:1000 | |||
| GCase | IB | Patient | MilliporeSigma, G4171 | 1:500 |
| GCase | IF | Patient | R&D Systems, MAB7410 | 1:250 |
| GFAP | IF | Patient/Mouse | Agilent, Z033429 | 1:1000 |
| HexA | IB | Patient | Santa Cruz Biotechnology, sc-376777 | 1:500 |
| HexA | IB | Mouse | Abcam, ab189865 | 1:500 |
| Iba1 | IF | Patient/Mouse | Wako, 019–19,741 | 1:500 |
| LAMP-1 | IB | Patient | Santa Cruz Biotechnology, sc-20011 | 1:1000 |
| LAMP-1 | IB | Mouse | Developmental Studies Hybridoma Bank, 1D4B | 1:100 |
| LAMP-2 | IB | Patient | Santa Cruz Biotechnology, sc-18822 | 1:1000 |
| MAP2 | IF | Patient | ThermoFisher, PA1-10,005 | 1:1000 |
| NeuN | IF | Patient | Abcam, ab177487 | 1:1000 |
| NeuN | IF | Mouse | MilliporeSigma, Abn91 | 1:500 |
| p-TDP-43 (Ser409/410) | IF | Mouse | Proteintech, 22,309–1-AP | 1:1000 |
| IHC | 1:60,000 | |||
| IB | Patient | 1:500 | ||
| SCMAS | IHC | Mouse | Abcam, ab181243 | 1:500 |
CatD cathepsin D, GCase β-glucocerebrosidase, HexA β-hexosaminidase A, IB immunoblot, IF immunofluorescence, IHC immunohistochemistry, SCMAS subunit C of mitochondrial ATP synthase