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. 2000 May;66(5):2252–2258. doi: 10.1128/aem.66.5.2252-2258.2000

FIG. 2.

FIG. 2

Coomassie blue-stained proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis during the purification of S. paucimobilis PgmG overproduced in cells of E. coli BL21(DE3) harboring plasmid pLC1 and induced with IPTG. Lanes: A, molecular markers in kilodaltons; B, crude extracts from cells of E. coli BL21(DE3) harboring plasmid pLC1 and harvested after 3 h of IPTG (0.1 mM) induction; C, fraction resulting from S-tag purification of PgmG.