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. 2000 May;66(5):2252–2258. doi: 10.1128/aem.66.5.2252-2258.2000

TABLE 2.

PGM and PMM activities in crude extracts from cells of E. coli BL21(DE3) harboring the cloning vector pET29a or the recombinant plasmid pLC1, with the S. paucimobilis pgmG gene, induced (3 h) or not induced with IPTG

Strain IPTG (mM) Mean ± SD sp act (U/g of protein) of:
PGM PMM
E. coli BL21(DE3)/pET29a 0.0 428.5 ± 18.5 52.7 ± 1.8
E. coli BL21(DE3)/pLC1 0.0 606.0 ± 14.0 68.3 ± 3.1
E. coli BL21(DE3)/pLC1 0.1 1,181 ± 18.5 123.0 ± 4.5