TABLE 2.
16S rRNA targeted oligonucleotide probes and experimental wash temperatures of buffer under experimental conditionsa
| Target group probe | Probe nameb | Primer sequence (5′ to 3′)c | Experimental wash temperature (°C) | Reference |
|---|---|---|---|---|
| Univ1390 | S-*-Univ-1390-a-A-18 | GACGGGCGGTGTGTACAA | 46 | 22 |
| Bact338 | S-D-Bact-0338-a-A-18 | GCTGCCTCCCGTAGGAGT | 54 | 2 |
| Bacto1080 | S-*-Bacto-1080-a-A-18 | GCACTTAAGCCGACACCT | 50 | 3 |
| Enter1432 | S-G-Enter-1432-a-A-15 | CTTTTGCAACCCACT | 43 | Chmieliewski et al., submitted for publication |
| Bif1412 | S-G-Bif-1412-b-A-21 | CGGGTGCTRCCCACTTTCATG | 52 | 8 |
| Lacto722 | S-G-Lacto-722-a-A-25 | YCACCGCTACACATGRAGTTCCACT | 51.2 | 17 |
| Clept1240 | S-G-Clept-1240-a-A-18 | GTTTTRTCAACGGCAGTC | 48.5 | This study |
| Erec482 | S-*-Erec-0482-a-A-19 | GCTTCTTAGTCARGTACCG | 47 | 5 |
a
Buffer was comprised of 1× SSC (0.15 M NaCl plus 0.015 M sodium citrate) and 1% sodium dodecyl sulfate.
b
Probe names are in accordance with the Oligonucleotide Probe Database (1).
c
Y represents a (C/T) wobble nucleotide and R represents an (A/G) wobble nucleotide.