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. 2023 Apr 29;71(5):725–734. doi: 10.1007/s12026-023-09385-1

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Proliferative capacity of cryopreserved individual and pooled PBMC preparations. Proliferative potential of PBMC (with or without stimuli) was determined by CFSE staining and flow cytometry analyses following the gating strategy shown in panel (A) Proliferative potential of two individual (I1 and I2) and two pooled PBMC preparations (pools of 3 and 5 donors, P3 and P5 respectively) were tested fresh and after thawing paired cryopreserved PBMC preparations (B) Basal proliferation of individual PBMC and pools is highlighted in B’ box, showing higher proliferation in pools both in fresh and after thawing cryopreserved PBMC. Absolute proliferation highlights the operating range for immunopotency assays