Fig. 3.

Stability of proliferation capacity of cryopreserved pools of PBMC. Cryovials from two pools of PBMC made from 5 donors each (in A, a pool composed of donor blood groups O⁺, A⁻, B⁺, A⁺, AB⁺; and in B, a pool composed of donor blood groups A⁺, O⁺, O⁺, O⁺, A.⁺) were thawed at different times (up to 13 and 15 months, respectively) and evaluated their capacity to proliferate in culture with and without polyclonal stimuli. Pool in A showed high capacity to respond to proliferation stimuli, whereas pool in B showed lower capacity to respond to proliferation stimuli. All measurements were performed on PBMC from cryovials thawed from either one of the two pools (A and B, respectively)