Table 1.

Comparison of composition and labelling efficiency in fresh and cryopreserved PBMC

%	CFSE labelling		Lymphocytes		Monocytes		Granulocytes
	Fresh	Cryo	Fresh	Cryo	Fresh	Cryo	Fresh	Cryo
Individual PBMC	99.4 ± 0.4	99.9 ± 0.0	54.3 ± 3.1	57.4 ± 8.6	4.7 ± 2.2	6.3 ± 2.9	10.6 ± 6.0	2.4 ± 0.5
Pooled PBMC	99.5 ± 0.2	88.5 ± 0.5	57.4 ± 16.8	70.5 ± 7.9	10.6 ± 7.9	5.6 ± 5.3	20.8 ± 23.2	8.0 ± 10.4

Individual and pools of 3 PBMC preparations were analysed for composition (%) of main cell subpopulations as well as efficiency for carboxyfluorescein succinimidyl ester (CFSE) labelling (%), expressed as mean ± standard deviation (n = 3). Please note that data presented is not from a donor-matched study

Cryo cryopreserved