Figure 6.

LKB1 overexpression corrects impaired hepatic autophagy in G6pt−/− mice. One-week-old control (+/+) and G6pt−/− (−/−) mice were treated with 2.5 × 10¹¹ pfu/kg of Ad-LKB1 and analyzed at age 3 weeks. Western-blot analyses were performed in untreated and Ad-LKB1-treated control and G6pt−/− mice (n = 6 for each group). (A) Western-blot analyses of hepatic LKB1, AMPK, p-AMPK, ULK1, p-ULK1 and β-actin. (B) Western-blot analyses of hepatic ATG101, Beclin1, Vps34, ATG3, ATG12-ATG5, LC3B, BNIP3, p62 and β-actin. Two sets of representative immune-stained lanes for untreated and Ad-SIRT1-treated control and G6pt−/− mice are shown for each protein. Densitometric quantification was performed and normalized against β-actin. Statistical analyses were performed between control and untreated or Ad-LKB1-treated G6pt−/− mice, and between untreated and Ad-LKB1-treated G6pt−/− mice. Values represent the mean ± SEM. ^*P < 0.05, ^**P < 0.01.