Table 1.
Overview of new Commensalibacter isolates, their isolation source and other isolation details. All isolates originated from Belgium. Insect samples were whole gut samples
| Source | N1 | Isolation medium2 | Isolation year | Strain |
|---|---|---|---|---|
| Bombus pascuorum | 3 | M13-A, M404-A | 2013 | LMG 28296 |
| Bombus hypnorum | 3 | AC–MA | 2013 | LMG 31819T and R-53529 |
| Vespa velutina 3 | 12 | M13–A, M404-A | 2019 | LMG 32512T |
| Sorbus aucuparia berries | 12 | MRS-A | 2020 | R-79671 to R-79674 |
| Aglais io | 1 | TSA–A | 2022 | R-83493 |
| Vespa velutina 3 | 3 | M13–A | 2022 | R-83526 |
| Pieris rapae 4 | 55 | M13–A, M404-A | 2022 | R-83534 |
| Vespa velutina 3 | 1 | M13–A | 2022 | R-83540 |
1Number of isolates with indistinguishable MALDI-TOF mass spectra
2AC, all culture agar (20 g/l tryptose, 3 g/l beef extract, 3 g/l yeast extract, 3 g/l malt extract, 5 g/l dextrose, 0.2 g/l ascorbic acid, 15 g/l agar); M13, LMG medium 13 (25% g/l D-mannitol, 5 g/l yeast extract, 3 g/l bacteriological peptone, 15 g/l agar)
M404, LMG medium 404 (50 g/l D-glucose; 10 g/l yeast extract, 15 g/l agar); MRS, Man, Rogosa and Sharpe agar (Oxoid); TSA, Tryptone Soya Agar (Oxoid); A, aerobic incubation; MA, microaerobic incubation. All isolation media comprised 10 ppm cycloheximide (Sigma-Aldrich) to inhibit fungal growth
3LMG 32512T, R-83526 and R-83540 were isolated from different V. velutina hornets each
4Gut samples from two P. rapae butterflies were analyzed