Table 5.

Summery of recovery and ionization loss in plasma, urine and feces. The recovery of the sample pretreatment was calculated from the ratio of the slopes of the pre-pretreatment spike over the post-pretreatment spiked samples. The ratio of the slopes of the post-pretreatment spike over the unprocessed academic calibration line resulted in thematrix effect by the biological matrix.

	Recovery			Matrix effect
	Plasma	Urine	Feces	Plasma	Urine	Feces
Compound	Average ± SD (%)	Average ± SD (%)	Average ± SD (%)	Average ± SD (%)	Average ± SD (%)	Average ± SD (%)
Curcumin	96.7 ± 16.7	74.1 ± 7.6	109.2 ± 38.5	12.9 ± 7.5	27.6 ± 21.8	15.8 ± 1.7
Demethoxycurcumin	96.8 ± 17.0	73.4 ± 16.2	106.2 ± 48.7	3.5 ± 10.3	49.9 ± 17.1	27.8 ± 15.7
Bisdemethoxycurcumin	101.5 ± 17.0	64.3 ± 13.5	111.1 ± 52.4	17.8 ± 2.6	56.3 ± 19.9	24.1 ± 24.2
Tetrahydrocurcumin	102.9 ± 28.0	101.5 ± 17.7	113.4 ± 25.2	−12.1 ± 46.1	51.6 ± 16.5	15.8 ± 8.9
Piperine-135	109.3 ± 0.8	101.2 ± 20.4	109.3 ± 44.7	4.7 ± 21.2	38.5 ± 15.1	5.5 ± 24.8