Figure 1.

Nucleotide metabolism is an enriched signature accompanying adipocyte differentiation.A, schematic of the experimental setup, the cell model, and sample preparation for steady-state metabolomics. B, intracellular abundance of metabolites profiled from 6 days of differentiated primary adipocytes relative to undifferentiated primary preadipocytes. C, table of the metabolic pathways significantly affected by 6-day differentiation. The pathway analysis module in MetaboAnalyst 5.0 was used for the analysis. D, schematic of the purine biosynthetic pathway illustrating metabolites that are depleted (red) or enriched (green) in 6-day differentiated primary adipocytes. E, relative levels of differentiation-enriched metabolites in the nucleotide biosynthesis pathway. Data shown are from four biological replicates. Statistical significance was determined using the Student’s t test. Error bars indicate mean ± SD, ∗p ≤ 0.05, ∗∗p ≤ 0.01, and ∗∗∗p ≤ 0.001.