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. 2023 Apr 17;62:102703. doi: 10.1016/j.redox.2023.102703

Fig. 2.

Fig. 2

Ferroptosis inducers inhibit TXNRD1 in cells. A) Cellular inactivation of TXNRD1 measured with the RX1 activity probe at 12 h; B) cellular inactivation of TXNRD1 measured with RX1 activity probe at 24 h; C) cellular inactivation of TXNRD1 measured with RX1 activity probe at 4 h. Similar suppression in RX1 fluorescence signal was seen at all timepoints when comparing incubation of cells with TRi-1 (C, right panel), RSL3 (A-C, left panels), or ML162 (A-C, middle left panel), but not with ML210 (A-C, middle right panel), at several doses as indicated; D) summary of activity as controlled for viability is shown for 4 h (left panel), 12 h (middle panel), and 24 h (right panel) incubation times. Viability data is presented in Extended DataFig. 3. Data are presented as mean ± s.d. of n = 3 biological replicates. Unpaired, two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 in comparison to DMSO control; non-significant differences are not noted.