Fig. 5.

Top: agarose gel electrophoresis images of pBR322 plasmid DNA incubated with 1, [complex] = 3.13 µM, using the light-exposure conditions P1–P4 (see main text for details), and without or with a ROS scavenger, viz. NaN₃ (¹O₂ quencher) and DMSO (HO^· scavenger). Lane 1: DNA control, lane 2: DNA irradiated with 1050–430 nm light for 30 min, lanes 3–6: complex samples prepared applying conditions P1–P4 without scavenger present, lanes 7–8: complex samples prepared as previously but with NaN₃ ([NaN₃] = 5 mM), lanes 11–14: complex samples prepared applying conditions P1–P4 with 10% DMSO. [DNA] = 15 µM (in base). Bottom: percentages of photocleavage without scavengers (blue), in the presence of 5 mM NaN₃ (green) and in the presence of 10% DMSO (red)