Table 1.
Antibodies Applied in Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry
| Antibody | Sources | Article Number | Working Concentration |
|---|---|---|---|
| TLR3 | ABclonal | A11778 | 1:1000 (WB) |
| TLR4 | ABclonal | A5258 | 1:2000(WB) |
| PI3K | Cell Signaling Technology | 17,366 | 1:1000(WB) |
| AKT | Cell Signaling Technology | 4691 | 1:1000(WB) |
| pAKT | Cell Signaling Technology | 4060 | 1:1000(WB) |
| Beta-actin | Cell Signaling Technology | 8457 | 1:1000(WB) |
| Gapdh | abcam | ab8245 | 1:1000(WB) |
| Mouse IgG HRP | Cell Signaling Technology | 7076 | 1:1000(WB) |
| Rabbit IgG HRP | Cell Signaling Technology | 58,802 | 1:1000 (WB) |
| TLR3 | ABclonal | A11778 | 1:1000 (IF) |
| Alexa Fluor Plus 594 | ThermoFisher | A21203 | 1:500 (IF) |
| Calreticulin | ABclonal | A18013 | 1:1000(FC) |
| PD-L1 | Biolegend | 124,312 | 1:50 |