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Establishment of bibenzyl backbone-producing strain E-DoBBS8. (a) The reconstructed biosynthetic pathway for bibenzyl backbones in E. coli; (b–d) HPLC analysis (λ = 280 nm) of the reaction catalyzed by E-DoBBS8 strain with malonic acid and S1 (b)/S2 (c)/S3 (d) as starting materials; HPLC analysis of (1) the standards S1–S3, (2) the negative reaction without substrate, (3) the reaction with malonic acid and S1–S3 as starting materials.
