Skip to main content
. 2022 Dec 14;13(4):1568–1587. doi: 10.1016/j.apsb.2022.12.008

Figure 1.

Figure 1

METRNL is highly present in the endothelium and secreted by endothelial cells. (A) METRNL in human and mouse blood vessels, indicated by arrows. Scale bar: 200 μm. (B) Metrnl mRNA in different mouse tissues (n = 5–8). WAT, white adipose tissue; BAT, brown adipose tissue. (C) METRNL and CD31 co-localized in mouse aortic endothelium. DAPI labels nuclei. Scale bar: 20 μm. Five independent experiments performed. (D) METRNL secretion from primary cultured mouse aortic endothelial cells (MAECs) in serum-free culture medium during 12 h (left, n = 3) and representative enzyme-linked immunosorbent assay (ELISA) standard curve for measuring mouse METRNL concentrations in cell culture medium or blood serum/plasma (right). (E) METRNL secretion from primary cultured human umbilical vein endothelial cells (HUVECs) in serum-free culture medium during 3 h (left, n = 6) and representative ELISA standard curve for measuring human METRNL concentrations in cell culture medium or blood serum/plasma (right). Data are mean ± SEM. ∗∗∗P < 0.001 by two-tailed Student's t test in (B, D and E).