Skip to main content
. 2022 Dec 14;13(4):1568–1587. doi: 10.1016/j.apsb.2022.12.008

Figure 3.

Figure 3

Endothelial METRNL deficiency reduces the circulating METRNL level. (A) Endothelial cell (EC)-specific Metrnl knockout (EC-Metrnl–/–) mice are generated by mating MetrnlloxP/loxP and Tek-Cre mice. (B) METRNL in the endothelium of aortae from EC-Metrnl–/– and wild-type (WT) mice. Scale bar: 20 μm. Four independent experiments performed. (C) Metrnl mRNA levels in mouse aortic endothelial cells (MAECs) (n = 6). (D) Metrnl mRNA levels in aortae with endothelium intact (n = 3–4). (E) Serum METRNL levels in EC-Metrnl–/– and WT mice (n = 16–17). (F) Protein and mRNA levels of METRNL in aortae and bone marrow (BM) of C57BL/6 and WT mice (n = 3–6). (G) Protein and mRNA levels of METRNL in BM of EC-Metrnl–/– and WT mice (n = 4–7). (H) Design of BM transplantation experiments (left), genotyping of BM DNA (middle), and Metrnl mRNA expression in BM of chimeric C57BL/6 mice receiving donor BM cells from EC-Metrnl–/– or WT mice (right) (n = 11–12). The recipient C57BL/6 mice using irradiation to destroy their own BM and then receiving donor BM cells from EC-Metrnl–/– or WT mice. (I) Serum METRNL levels in chimeric C57BL/6 mice receiving donor BM cells from EC-Metrnl–/– or WT mice (n = 11). +: normal expression of Metrnl; –: deficient expression of Metrnl. Data are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 by two-tailed Student's t test in (C, E, F right, G, and H) and Mann–Whitney U test in (D). ∗∗∗P < 0.001 vs. C57 Aorta by one-way ANOVA followed by Dunnett's t test in (F left).