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. 2022 Dec 14;13(4):1568–1587. doi: 10.1016/j.apsb.2022.12.008

Figure 8.

Figure 8

Endothelial METRNL deficiency aggravates endothelial inflammation during atherosclerosis. (A–C) mRNA expressions of inflammatory markers in aortae from EC-Metrnl–/– mice (A, n = 6–9), ApoE–/–EC-Metrnl–/– mice at 3 months of age under normal chow without atherosclerosis (B, n = 9–11) and ApoE–/–EC-Metrnl–/– mice fed 22 weeks of HFD with obvious atherosclerosis (C, n = 4–6) as well as their littermates. (D) Immunohistochemical staining for F4/80 in aortic roots from ApoE–/–EC-Metrnl–/– and ApoE–/–WT mice after 22 weeks of HFD (n = 5). Bar = 500 μm. (E, F) mRNA expressions of inflammatory markers in METRNL deficient HUVECs and controls under stimulation of oxLDL (100 μg/mL) (E, n = 6) or LPS (1 μg/mL) (F, n = 5–6) for 24 h. LDL, low density lipoprotein; oxLDL, oxidized low density lipoprotein; LPS, lipopolysaccharide; VCAM, vascular cell adhesion molecule; ICAM, intercellular adhesion molecule; TNF, tumor necrosis factor; IL, interleukin; MCP, monocyte chemoattractant protein; CD, cluster of differentiation; E-SEL, E-selectin. Data are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 by two-tailed Student's t test in (C, D and F). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. Scrambled shRNA + LDL; ##P < 0.01, ###P < 0.001 vs. Scrambled shRNA + oxLDL by one-way ANOVA followed by Dunnett's t test in (E).