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. 2023 Apr 17;14:1112610. doi: 10.3389/fphar.2023.1112610

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Copyright © 2023 Rao, Su, Zhang, Wang, Li, Li, Zeng and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibitory effects of HJ on IPI-FX cells infected with PEDV virus at different stages. (A) IPI-FX cells were treated with HJ at different times before or after infection. Double-headed red arrows indicate the presence of HJ, while the orange horizontal lines indicate the time of PEDV infection. The experiments are identified in the text by the numbers on the left (M0–M7). (B) The expression level of PEDV N protein in IPI-FX cells was examined by western blot assays. Cells were harvested for 12 h. The expression level of PEDV N protein was calculated in relation to the expression level of GAPDH. Results are representative of three independent experiments. Data are represented as mean ± SD, n = 3. *, and p < 0.05 was considered statistically significant compared with M1, while ^#, and p < 0.05 was considered statistically significant compared with M2.