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Effect of desensitization to one stimulus on response to a second stimulus. Cells were desensitized to anti-IgE (3 μg/ml) or to protein A (200 nM) by preincubation with the stimuli in Ca2+-free P-EDTA for 30 min at 37°C. Cells were then washed twice at 4°C, resuspended in PCG, and challenged with anti-IgE (3 μg/ml) or protein A (200 nM) for 30 min at 37°C. Each bar shows the mean ± SEM. Error bars are not shown when graphically too small.
