FIG. 6.

Effect of lactic acid treatment on histamine release from HHMC induced by protein A, anti-IgE, and anti-FcɛRIα. HHMC were either untreated (buffer) or treated with lactic acid (0.01 M, pH 3.9, 5 min, 22°C) and washed twice. Cells were then challenged with protein A (200 nM), anti-IgE (3 μg/ml), or anti-FcɛRIα (1 μg/ml) for 30 min at 37°C. Each bar shows the mean of duplicate determinations of a typical experiment. Similar results were obtained in two other experiments.