Figure 1.

Expression and binding of PD-1 antibody. (A) Schematic of DNA construct encoding PD-1 antibody. The α- PD-1-Fc gene was cloned onto the pVAX vector and driven by the CMV promoter. (B) Structure of PD-1 antibody. α-PD-1-Fc contained scFv of mouse PD-1 antibody and the Fc region of mouse IgG1 with a D265A mutation. (C) Western blot analysis of mouse IgG from supernatants of pVAX-α-PD-1-Fc transfected 293T under non-reduced conditions. The pVAX-empty vector served as a control. Numbers indicate molecular weight. (D) The binding ELISA of α-PD-1-Fc from cell culture supernatants with mouse PD-1 antibodies. Results are representative means ± SDs of three independent experiments.