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. 2023 Apr 17;14:1169850. doi: 10.3389/fimmu.2023.1169850

Figure 4.

Figure 4

Lung metastasis inhibition of combination DNA vaccination and intramuscular gene transfer of PD-1 antibody in B16F10-OVA model. (A) Schematic protocol of the B16F10-OVA inoculation and combination therapy of DNA vaccination with pVAX-α-PD-1-Fc. C57BL/6 mice were divided into four groups: the combination of pOVA vaccine with α-PD-1 antibody (pCMV-OVA + α-PD-1 and pEMS-OVA + α-PD-1), the combination of vaccine without L/E/G and α-PD-1 (pCMV-OVA-none + α-PD-1) and no vaccine (empty vector). N = 5 per group. (B) Images of lung metastasis taken 2 weeks after B16F10-OVA inoculation. Number of metastases nodules on the surface of lung (C) and the wet weight of lungs (D) of the four groups. (E) Relative body weight change of mice receiving four different treatments. The data (C–E) represent the means ± SDs and were subjected to a one-way ANOVA; “NS” indicates no significant difference. *p < 0.05; **p < 0.01. ANOVA, analysis of variance; pluronic L64 (L)/electroporation (E)/epigallocatechin gallate (G) (EGCG) (referred to as L/E/G).