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. 2023 Apr 17;14:1169850. doi: 10.3389/fimmu.2023.1169850

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Copyright © 2023 Liu, Yang, Zheng, Liu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immune cell populations and biochemical analysis of combination DNA vaccination and intramuscular gene transfer of PD-1 antibody in B16F10-OVA model. All mice were sacrificed at day 29. Immune cell populations analysis of spleen and blood from mice receiving four different treatments. (A) Percentage of CD4⁺ T cells in the blood. (B) Percentage of CD8⁺ T cells in the blood. (C) Percentage of CD4⁺ T cells in the spleen. (D) Percentage of CD8⁺ T cells in the spleen. (E) Spleen weight. (F) ALT level in the serum of mice. (G) AST level. Data are shown as means ± SDs. Statistical analyses were performed using one-way ANOVA with Dunnett’s multiple comparison test. *p < 0.05; **p < 0.01; ***p < 0.001. ANOVA, analysis of variance; AST, aminotransferase; ALT, alanine aminotransferase. .