Figure 3.
Genetic analysis of CACNA1C-G37R and CACNB2b-S143F. (a) Electropherogram of CACNA1C-WT and CACNA1C-G37R and amino acid sequence alignment of CACNA1C-G37R. (b) Electropherogram of CACNB2b-WT and CACNB2b-S143F and amino acid sequence alignment of CACNB2b-S143F. (c) Schematic of the CaV1.2 channel pore-forming α1C subunit and the auxiliary α2δ and β subunits. The CACNA1C-G37R mutation is in the N-terminus close to the PKA binding site (black circle). The CACNB2b-S143F mutation is located at the Hook region. Phosphorylation sites by PKA and calcineurin (CaN) binding sites located in CaV1.2 and β subunits (white cycles with P). BID, β subunit interacting domain and AID, α subunit interacting domain. (Online version in colour.)