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eCB levels as measured by LC-MS/MS. One half of the spleen (A) and lung (B, C) from mixed-sex neonatal (C) and adult female (A, B) WT and Cnr1−/− mice was extracted for eCBs and quantified using deuterated standards (n=3–5). The resulting values were standardized by tissue weight except in the adult lungs. Adult lung values are expressed as the ratio of eCB to deuterated standard. Data are expressed as mean ± SD, *p<0.05 for indicated comparison.
