Adaptive immune cell phenotypes by flow cytometry. Cells 379 immune cell from WT and Cnr1−/− mixed-sex neonatal spleens (A, n=3–5) were stained for extracellular markers to identify T helper cells (CD4), 380 populations and the cytotoxic T cells (CD8), and B cells (CD19). Additionally, cells from WT and Cnr1−/− adult female spleens (B, n=4–9), and lung (C, n=4–5) potential role for CB1 were similarly stained. Data are expressed as the mean of percent lymphocyte gated ± SD. None were significant within cell surface 382 was examined. CPF marker group.