2-AG hydrolytic activity and IL-6 ELISA of treated adult splenocytes. Murine splenocytes were following a 24 h incubation isolated from naïve mice and treated with vehicle, LPS (1 μg/mL), CPO (1 μM), or a combination of LPS and period with LPS. To explore the CPO. After 3.5 hr, the media and cells were harvested together to measure 2-AG hydrolytic activity by LC-MS/MS (A) and IL-6 levels by ELISA (B), respectively. Data are expressed as mean ± SD (n=3 mice & 3 were performed with and without technical replications), *p<0.05 for indicated comparison.