Cytokine ELISA results for splenocytes treated with DMSO or a CB1 antagonist. Splenocytes were isolated from naïve adult female (A) and male (B) mice. Cells from each mouse were first divided in half and treated with SR141716A (CB1 Ant) in DMSO or DMSO. Once plated, cells were treated additionally with CPO (1 μM) in EtOH or EtOH (Veh) ± LPS (1 μg/mL) for 24 hr at 37°C. IL-1β, IL-6, TNF-α, and IFN-γ levels were measured in the serum by ELISA. Data are expressed as mean ± SD (n=3), *p<0.05 for indicated comparison.