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Cytokine ELISA results for splenocytes from WT and Cnr1−/− littermates. Splenocytes were isolated from wild-type (WT) and Cnr1−/− female and male murine littermates (n=3). Cells were left untreated or treated with 0.1 μg/mL or 1 μg/mL LPS for 24 h (D1), 48 h (D2), or 72 h (D3). IL-1β (A), IL-6 (B), TNF-α (C), and IFN-γ (D) levels were measured in the serum by ELISA. Data are expressed as mean ± SD (n=4 technical replications), *p<0.05 for indicated comparison.
